Differences in Plasma Glucose as Measured by Glucose Oxidase and Hexokinase Laboratory

South Africa, 2016 - 2017

Lynnsay M. Dickson, Eckhart J Buchmann, Charl Janse Van Rensburg, Shane A. Norris

Identification

Survey ID Number

DifferencesinPlasmaGlucoseasMeasuredbyGlucoseOxidaseandHexokinaseLaboratory

Title

Differences in Plasma Glucose as Measured by Glucose Oxidase and Hexokinase Laboratory

Country

Name
South Africa

Abstract

We evaluated the extent of measurement discordance between glucose oxidase and hexokinase laboratory methods and the effect of this on estimated gestational diabetes mellitus (GDM) prevalence in a routine clinical setting. 592 consecutive urban African women were screened for GDM. Paired venous specimens were submitted to two independent calibrated laboratories that used either method to measure plasma glucose concentrations. World Health Organisation diagnostic criteria were applied. GDM prevalence determined by the glucose oxidase and hexokinase methods was 6.9% and 5.1% respectively. Only 34% of GDM positive diagnoses were common to both laboratory methods. Bland Altman plots identified a bias of 0.2 mmol/l between laboratory methods. Plasma glucose concentrations measured by the glucose oxidase method were more platykurtic in distribution. Low diagnostic agreement between laboratory methods was further indicated by a Cohen's kappa of 0.48 (p <0.001). Reports of GDM prevalence using either the glucose oxidase or hexokinase laboratory methods may not be truly interchangeable or directly comparable.

Producers and sponsors

Primary investigators

Name	Affiliation
Lynnsay M. Dickson	MRC Developmental Pathways to Health Research Unit, Department of Paediatrics, Faculty of Health Sciences, University of the Witwatersrand, York Road, Parktown, Johannesburg, South Africa
Eckhart J Buchmann	Department of Obstetrics and Gynaecology, Faculty of Health Sciences, University of the Witwatersrand, York Road, Parktown, Johannesburg, South Africa
Charl Janse Van Rensburg	Biostatistics Unit, South African Medical Research Council, 1 Soutpansberg Road, Pretoria, South Africa
Shane A. Norris	MRC Developmental Pathways to Health Research Unit, Department of Paediatrics, Faculty of Health Sciences, University of the Witwatersrand, York Road, Parktown, Johannesburg, South Africa

Data Collection

Dates of Data Collection

Start	End
2016-04-01	2017-05-31