Differences in Plasma Glucose as Measured by Glucose Oxidase and Hexokinase Laboratory Nesstar Publisher Differences in Plasma Glucose as Measured by Glucose Oxidase and Hexokinase Laboratory DifferencesinPlasmaGlucoseasMeasuredbyGlucoseOxidaseandHexokinaseLaboratory Lynnsay M. Dickson Eckhart J Buchmann Charl Janse Van Rensburg Shane A. Norris Nesstar Publisher We evaluated the extent of measurement discordance between glucose oxidase and hexokinase laboratory methods and the effect of this on estimated gestational diabetes mellitus (GDM) prevalence in a routine clinical setting. 592 consecutive urban African women were screened for GDM. Paired venous specimens were submitted to two independent calibrated laboratories that used either method to measure plasma glucose concentrations. World Health Organisation diagnostic criteria were applied. GDM prevalence determined by the glucose oxidase and hexokinase methods was 6.9% and 5.1% respectively. Only 34% of GDM positive diagnoses were common to both laboratory methods. Bland Altman plots identified a bias of 0.2 mmol/l between laboratory methods. Plasma glucose concentrations measured by the glucose oxidase method were more platykurtic in distribution. Low diagnostic agreement between laboratory methods was further indicated by a Cohen's kappa of 0.48 (p <0.001). Reports of GDM prevalence using either the glucose oxidase or hexokinase laboratory methods may not be truly interchangeable or directly comparable. South Africa GDMdata.NSDstat 592 34 Nesstar 200801 part_no 592 0 AB19860114 1 AC19800116 1 AC19971212 1 AJ19850513 1 AK19801218 1 AM19760105 1 AM19830418 1 AM19830621 1 AM19831114 1 AM19861220 1 AM19871230 1 AM19920427 1 AM19930212 1 AM19940626 1 AN19780625 1 AN19910309 1 AN19910803 1 AN19971028 1 AS19920910 1 AW19881001 1 BD19780413 1 BD19950725 1 BG19821125 1 BG19890313 1 BG19931122 1 BH19841010 1 BK19930628 1 BL19860626 1 BM19830831 1 BM19860916 1 BM19890222 1 BM19911027 1 BM19950629 1 BM19951220 1 BM19970607 1 BN19850930 1 BN19860512 1 BN19861203 1 BN19890922 1 BN19950114 1 BS19940917 1 BT19870112 1 BX19791206 1 BZ19860512 1 BZ19870617 1 CD19970108 1 CK19860710 1 CM19791125 1 CM19840107 1 CM19900428 1 CM19931012 1 CM19941201 1 CN19990319 1 CT19820323 1 DB19970904 1 DC19940716 1 DL19930427 1 DM19790529 1 DM19861106 1 DM19890717 1 DM19911119 1 DM19911209 1 DN19820630 1 DN19860728 1 DN19941205 1 DR19881004 1 DS19940413 1 DT19981215 1 DX19900520 1 EB19920404 1 EC19840919 1 EG19870724 1 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TS19961010 1 TT19900817 1 TV19890908 1 TX19840321 1 TY19820625 1 TZ19911004 1 TZ19950702 1 UM19970513 1 VA19901212 1 VM19920207 1 VM19930616 1 VN19860107 1 VN19950803 1 XG19870607 1 XM19850901 1 XN19780404 1 XS19820306 1 XZ19860321 1 YD19890422 1 YM19751205 1 YS19900611 1 ZB19750919 1 ZD19920707 1 ZD19940912 1 ZH19870511 1 ZH19970308 1 ZJ19820831 1 ZJ19830405 1 ZK19820808 1 ZK19831002 1 ZK19870714 1 ZK19891208 1 ZM19791012 1 ZM19831229 1 ZM19861206 1 ZM19870717 1 ZM19930324 1 ZM19940519 1 ZM19960216 1 ZM19960510 1 ZM19960615 1 ZN19820710 1 ZN19851022 1 ZN19870111 1 ZN19930222 1 ZN19971110 1 ZN19980510 1 ZS19730403 1 ZS19791116 1 ZS19811207 1 ZS19830205 1 ZS19920805 1 ZS19980329 1 ZT19811018 1 ZT19851014 1 ZT19940110 1 ZX19940420 1 ZZ19890714 1 gox_t0 592 0 2.49 8.34 4.006 0.751 gox_t60 592 0 2.19 14.16 5.417 1.506 gox_t120 592 0 2.16 16.29 5.117 1.37 HK_t0 592 0 3 8.8 4.126 0.487 HK_t60 592 0 2.3 13.5 5.621 1.389 HK_t120 592 0 2.7 14.7 5.268 1.208 abn_gox_t0 592 0 0 556 1 36 abn_gox_t60 592 0 0 584 1 8 abn_gox_t120 592 0 0 583 1 9 abn_HK_t0 592 0 0 568 1 24 abn_HK_t60 592 0 0 585 1 7 abn_HK_t120 592 0 0 581 1 11 age 592 0 18 47 27.775 5.892 smoker 592 0 NO 571 YES 21 chronHT 591 0 NO 572 YES 19 PrevGDM 592 0 NO 589 YES 3 Gest_visit1 592 0 3 35 19.139 5.598 Glycosuria 592 0 NAD 586 YES 6 Weight 592 0 40.8 128 70.634 15.759 Height 592 0 116 187 161.886 7.197 MUAC 592 0 19.5 52.5 29.877 4.42 S_BP 591 1 26 182 122.176 14.871 D_BP 591 1 17 108 72.565 10.948 Gravida 592 0 1 173 2 196 3 144 >3 79 PrevLGA 591 0 NO 548 YES 43 nPrevMis 592 0 0 527 1 60 2 5 nPrevStillb 592 0 0 560 1 27 1 twins 1 2 3 3 1 nPrevCongenAbN 591 1 0 591 Sysmiss 1 nPrevStillb_number 591 1 0 559 1 28 2 3 3 1 Sysmiss 1 gdm_gox 592 0 0 551 1 41 gdm_HK 592 0 0 562 1 30 FHxDM 588 0 1st degree 99 2nd degree 92 None 397 bmi 592 0 15.93 86.43 27.014 6.306